Sterilizing and sanitizing solutions are used in plant tissue culture:
Various methods or chemicals are used for these purposes, including:
Other sterilants are infrequently used in home plant tissue culture (if ever), but are used for commercial sterilization:
Most of these chemical agents act through a process of general oxidation. In other words, the action is almost non-specific to any organism, but is based on the agent attacking the cell wall through oxidation, leading to its rupture and the death of the cell through this cause and residual oxidation. Stronger oxidants release more energy per molecular interaction, and can therefore destroy stronger bonds. They often go together with shorter exposure times so as to not destroy wanted explant cells.




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 Copyright 2011+ Arthur Sale
Last Modified: 20 January 2011